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VizPrimer:基于可视化基因结构的引物设计软件  

2011-10-19 21:23:13|  分类: Software |  标签: |举报 |字号 订阅

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见网址:http://biocompute.bmi.ac.cn/CZlab/VizPrimer//

Bioinformatics. 2011 Oct 20. [Epub ahead of print]

VizPrimer: a web server for visualised PCR primer design based on known gene structure.

Source

Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850; College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064; Beijing Institute of Health Service and Medical Information, Beijing 100850, China and School of Life Science, Anhui Medical University, Anhui, 230032.

Abstract

SUMMARY: The visualisation of gene structure plays an important role in PCR primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualised web server for primer design named VizPrimer. It utilises the new IT (Information Technology) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs). AVAILABILITY: VizPrimer is freely available at http://biocompute.bmi.ac.cn/CZlab/VizPrimer/. (Supported browsers: Chrome [?5.0], Firefox [?3.0], Safari [?4.0], Opera [?10.0]).Contacts: CZ (zhangcg@bmi.ac.cn); YY (yangyi528@vip.sina.com).

PMID:
 
22016409
 
[

Review的评价很到位:

VizPrimer is proposed as in interactive web server tool for non-expert bioinformaticians and biologist to explore gene structure and its isoforms of a gene of interest. It also helps design primers to isolate particular isoform(s) using simple and smooth set of operations with aesthetic graphical display. 
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